Immunochemical Characterization of Porphyridium cruentum B-Phycoerythrin: Proof of Cross-Reaction between Chromophore-Free Apoprotein and Holoprotein-Specific Antibodies
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چکیده
Antibodies against phycobilisom es o f Porphyridium cruentum immunoreact with B-phycoerythrin from the same organism. A strong antigen-antibody com plex is not only formed with the native B-phycoerythrin but also when the chromophore is chemically reduced, split or even degraded with chromic acid. Characteristics o f precipitations and dissolutions in the presence of the chaotropic reagents urea and potassium rhodanide are discussed for the immunocomplex with native B-phycoerythrin. A sandwich-type immunoadsorbent column appropriate for antigen-isolation is described; its binding properties are investigated biochem ically and by electron microscopy.
منابع مشابه
Recovery of pure B-phycoerythrin from the microalga Porphyridium cruentum.
Phycoerythrin is a major light-harvesting pigment of red algae and cyanobacteria that is widely used as a fluorescent probe and analytical reagent. In this paper, B-phycoerythrin and R-phycocyanin in native state, from the red alga Porphyridium cruentum were obtained by an inexpensive and simple process. The best results of this purification procedure were scaled up by a factor of 13 to a large...
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